Dynamic organization of actin cytoskeleton during the polarity formation and germination of pollen protoplasts中国科学通报：英文版论文
【题名】：Dynamic organization of actin cytoskeleton during the polarity formation and germination of pollen protoplasts中国科学通报：英文版论文(Dynamic organization of actin cytoskeleton during the polarity formation and germination of pollen protoplastsZhongGuoKeXueTongBao：YingWenBanLunWen) 【关键词】：肌动蛋白细胞骨架 花粉 原生质体 极性 气孔 【keywords】：JiDongDanBaiXiBaoGuJia HuaFen YuanShengZhiTi JiXing QiKong 【作者】：XUXia,ZIHuijun,SUNYina,RENHaiyun,      【来源】： 知识词典 【期刊名称】：中国科学通报：英文版(ZhongGuoKeXueTongBao：YingWenBan) 【国际标准刊号】：1001-6538        【国内统一刊号】：11-1785/N 【作者单位】：[1]CollegeofLifeScience,BeijingNormalUniversity,Beijing100875,China [2]TheStateKeyLaboratoryofPlantPhysiologyandBiochemistry,ChinaAgriculturalUniversity,Beijing100094.China([1]CollegeofLifeScience,BeijingNormalUniversity,Beijing100875,China [2]TheStateKeyLaboratoryofPlantPhysiologyandBiochemistry,ChinaAgriculturalUniversity,Beijing100094.China) 【分类号】：Q944.42      【页码】：-1702-1706      【出版年】：2004.16 【下载地址】：下载pdf全文         The formation of the polarity of pollen protoplast and the dynamics of actin cytoskeleton were observed by non-fixation, Alexa-Phalloidin probing and confocal laser scanning microscopy. Our results showed that the protoplast obtained from stored pollen contained numerous crystalline fusiform bodies to constitute a storage form of actin. When dormant pollen was hydrated, the actin cytoskeleton forms a fine network spreading uniformly in the protoplast. In the process of polarity formation and germination of pollen protoplast, actin filaments marshaled slowly to the brim, and then formed multilayer continuous actin filament bundles surrounding the cortical of the protoplast. When the protoplast was exposed to actin filament-disrupting drugs, such as Latrunculin A and Cytochalasin D, continuously arranged actin bundles were disturbed and in this condition, the protoplast could not germinate. But when exposed to actin filament stabiling drug-phalliodin, the dynamics of actin filaments in the protoplasts behaved normally and the protoplasts could germinate normally. These results were also confirmed by the pharmacology experiments on pollen grains. And when Latrunculin A or Cytochalasin D was washed off, the ratio of pollen germination was resumed partly. All the results above show that the dynamic organization of the actin cytoskeleton are critical in the cell polarity formation and germination of pollen protoplast, and that the reorganization of actin cytoskeleton is mainly due to the rearrangement of actin filament arrays.
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