Study on Immunochemical Assays for the Organophosphorus Insecticide Chlorpyrifos中国农业科学：英文版论文

Study on Immunochemical Assays for the Organophosphorus Insecticide Chlorpyrifos中国农业科学：英文版论文

【题名】：Study on Immunochemical Assays for the Organophosphorus Insecticide Chlorpyrifos中国农业科学：英文版论文(Study on Immunochemical Assays for the Organophosphorus Insecticide ChlorpyrifosZhongGuoNongYeKeXue：YingWenBanLunWen)
【关键词】：有机磷杀虫剂毒死蜱免疫化学测定抗体
【keywords】：YouJiLinShaChongJi DuSiZuo MianYiHuaXueCeDing KangTi
【作者】：WUGang,HUANGYa-li,ZHUGuo-nian,WUHui-ming,LICong,      【来源】：知识词典
【期刊名称】：中国农业科学：英文版(ZhongGuoNongYeKeXue：YingWenBan)
【国际标准刊号】：1671-2927        【国内统一刊号】：11-4720/S
【作者单位】：[1]ZhejiangEntry-ExitInspection＆QuarantineBureau,Hangzhou310012,P.R.China [2]InstituteofPesticide＆EnvironmentalToxicology,ZhejiangUniversity,Hangzhou310029,P.R.China([1]ZhejiangEntry-ExitInspection＆QuarantineBureau,Hangzhou310012,P.R.China [2]InstituteofPesticide＆EnvironmentalToxicology,ZhejiangUniversity,Hangzhou310029,P.R.China)
【分类号】：S482.33      【页码】：-371-375      【出版年】：2004.5
        The anti-chlorpyrifos polyclonal antibodies were obtained by using the artificial immune antigen to immune in New Zealand's white rabbits. The enzyme-tagged antibodies were prepared by coupling horseradish peroxidase (HRP) to the purified antibody with the modified sodium periodate method. The indirect competitive enzyme linked immuno-sorbent assays (ELISA) and the HRP-tagged antibody direct ELISA (E-Ab) were established, respectively. The limit of detection (LOD) for the indirect ELISA and E-Ab were 0.0033 and 0.0042 μg mL^-1, respectively. The linear detection ranged well from 0.005 to 2.0 μg mL^-1.

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